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. 2016 Jun 3;26(7):775–786. doi: 10.1038/cr.2016.66

Figure 5.

Figure 5

MutSβ-Polβ complex colocalizes with (CAG)•(CTG) repeats during DNA synthesis. (A) Confocal immunofluorescence analysis showing MSH3 and Polβ foci and their colocalization in HeLa cells in G1 phase (top) and S phase (bottom). (B) DNA sequence of 5 end Cy3-labeled oligonucleotide used in FISH analysis and its mapping site at the ectopic c-Myc replication origin in HeLa-(CAG)45 cells. (C, D) Confocal immunofluorescence analysis showing MSH3-(CTG)45 and Polβ-(CTG)45 colocalizations in HeLa-(CAG)45 cells in S phase, respectively. (E) Confocal immunofluorescence analysis showing colocalization of MSH3, Polβ and (CTG)45 in HeLa-(CAG)45 cells in S phase (bottom), with HeLa cells without the ectopic (CAG)45 sequence as a negative control (top). β and 3 represent Polβ and MSH3, respectively. (F) Percentage of cells showing colocalization of MSH3, Polβ and (CTG)45 repeats.