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HepaRG progenitor cells were cultured with control FCS(−)/BSA(+) or FCS(−)/BSA(+)/Wnt3a. A. HAPLN1 mRNA levels 12 h and 13 days upon Wnt3a-induction. B. Immunodetection of HAPLN1, red (Cy5); N-cadherin, green (FITC). Nuclei, blue (DAPI). Images acquired at 20X. Control (BSA) shows very rare cells expressing HAPLN1 (arrow). HAPLN1 labels the pericellular matrix and colocalizes with N-cadherin.
