Table 5. Primers used for the APOE PCR and the ARMS PCR for rs4925.

primer	Sequence	Tm (°C)	Conc. (μM)
APOE-FW	GACGCGGGACGGCTGTCCAAGGAGCTGCAGG CGACGCAGGCCCGGCTGGACGCGGACATGGA GGA	99.6	1.0
APOE-RV	AGGCCACGCTCGACGCCCTCGCGGGCCCCGGCCTGGTACACT	90.7	1.0
GSTO1-FWinnerA	TATTAGAAGCCAAAATAAAGAAGACTACGA	56	0.90
GSTO1-RVouter	GAAAGTGGGAATATAAAGAAAAGAATAGGA	56	0.60
GSTO1-FWouter	CGATACAGTTAGCCATAAACTGATAAACTAA	56	0.60
GSTO1-RVinnerC	ATTCTTTACGAAATTCTTCTTTTAGGCTAG	56	0.84

The resulting fragments for the APOE genotyping are explained in Methods. The ARMS PCR for rs4925 generates a total fragment of the two outer primers (239bp) that serves as a positive control, a major C allele-specific fragment (132bp) and/or a minor A allele-specific fragment (167bp). All sequences are given in the 5′ to 3′ direction.

Abbreviations: Tm… melting temperature, Conc. … concentration, μM… 10E-6 mol/L