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. 2016 May 9;7(26):39184–39196. doi: 10.18632/oncotarget.9258

Figure 6. Delay in neuronal migration by Prrt2 knockdown during development.

Figure 6

A. Coronal sections of the mouse brains 3 and 5 days after in utero electroporation of shControl or shPrrt2 constructs at E14.5. Three days after electroporation, cells expressing EGFP (green) and shControl had started migration, with many cells having already reached the CP. More cells were restricted to the VZ and IZ in the brains electroporated with each of the shPrrt2. This migration delay was rescued by co-transfection of shPrrt2 and Prrt2 constructs (upper panel). When brain sections were examined 5 days post electroporation, most cells in both shControl and shPrrt2 groups had reached the CP (lower panel). All sections were stained with DAPI (blue) to show the cell nuclei. Scale bars = 100 μm. B. Statistical analysis showed significant differences in cell distribution in the CP, IZ and VZ at E17.5for the four conditions. Error bars represent SEM; *: p < 0.05, ** : p < 0.01.