Figure 1. EAC exhibits intact basal autophagy in vitro as determined by immunoblotting of markers LC3B and p62 upon pharmacological autophagy inhibition.

A. EAC cell lines OE19 and OE33 were treated with increasing concentrations of the autophagy inhibitor chloroquine (CQ) for 48hr followed by immunoblotting of the autophagy markers p62 and LC3B. Total protein was visualized as loading control. Representative blot is shown. B. Quantification of triplicate repetitions of experiments shown in A.