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. 2016 Jun 14;7(26):39332–39344. doi: 10.18632/oncotarget.10045

Figure 4. The effect of a HF diet on rat pituitary-adrenal axis hormone levels.

Figure 4

Rats were fed a NC diet or a HF diet for 28 weeks. A. Acth gene expression. B. The total pituitary content of ACTH was measured by ELISA and corrected against the total protein content. C. Representative images of ACTH immunohistochemical staining are shown (magnification, × 200). All positive immunostaining is indicated by brown in the cytoplasm. D. Serum ACTH and COR levels at 0, 4 or 28 weeks. The data are expressed as the mean ± SD (n = 20 per group). * p < 0.05, ** p < 0.01 versus the corresponding NC group.