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. 2016 May 18;7(26):39680–39693. doi: 10.18632/oncotarget.9444

Figure 1. ERBB2 overexpression enhances tumorigenesis and radiation resistance to cause poor prognosis in ESCC.

Figure 1

(A) Immunohistochemistry was conducted to detect the expression of ERBB2 in ESCC tumor samples. Scale bar, 200 μm. Kaplan-Meier plots of overall survival in 131 ESCC patients stratified by ERBB2 expression level. (B) Western blotting showed endogenous ERBB2 expression in normal human esophageal epithelial cells and KYSE cells. (C) Western blotting showed the expression of ERBB2 in ERBB2-down-regulated stable cell lines (KYSE70 and KYSE510) and an ERBB2-up-regulated stable cell line (KYSE170). (D and E) The colony formation assay was used to analyze ERBB2-dependent tumorigenesis in vitro; *P < 0.05; **P < 0.01. (F) The survival fraction was used to represent radiosensitivity in KYSE cells; *P < 0.05; **P < 0.01; ***P < 0.001.