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. 2016 May 18;7(26):39680–39693. doi: 10.18632/oncotarget.9444

Figure 2. Down-regulation of ERBB2 is regulated by miR-193a-5p to enhance radiosensitivity.

Figure 2

(A) Bioinformatics websites and a human microarray data were employed to predict miRNAs that regulate ERBB2 expression. (B) Western blotting showed ERBB2 expression after manipulating miR-193a-5p in KYSE cells. (C and D) The luciferase reporter assay was used to demonstrate direct binding between the seed region (SR) of miR-193a-5p and ERBB2-3′UTR or ERBB2-Mut 3′UTR; *P < 0.05; **P < 0.01. (E) A colony formation assay was used to verify the involvement of miR-193a-5p in ERBB2-dependent tumorigenesis in vitro; *P < 0.05; ns, non-significant. (F) The survival fraction was used to represent radiosensitivity in KYSE cells; *P < 0.05; ***P < 0.001.