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. 2016 May 18;7(26):39680–39693. doi: 10.18632/oncotarget.9444

Figure 3. Up-regulation of miR-193a-5p decreases ERBB2 expression level to suppress ESCC tumorigenesis in vitro and in vivo.

Figure 3

(A) Real-time PCR was used to evaluate miR-193a-5p expression in HET-1A cells and KYSE cells. ERBB2 was inversely correlated with miR-193a-5p expression in KYSE cells; R2 = – 0.666; P = 0.04. (B) A miR-193a-5p precursor was transfected into KYSE70 and KYSE510 cells; ***P < 0.001. MiR-193a-5p inhibitors were transfected into KYSE170 cells; **P < 0.01. (C and D) The effect of miR-193a-5p on tumorigenesis in vitro was analyzed using a colony formation assay in KYSE cells; *P < 0.05; **P < 0.01. (E) A xenograft mouse model was used to analyze the effect of miR-193a-5p on tumorigenesis in vivo; *P < 0.05. (F) Immunohistochemistry was used to verify the expression of ERBB2 in xenograft tumors. Scale bar, 500 μm (40×); 50 μm (200×).