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. 2016 May 18;7(26):39680–39693. doi: 10.18632/oncotarget.9444

Figure 4. Down-regulation of miR-193a-5p is inversely correlated with ERBB2 expression level and is correlated with poor prognosis in ESCC.

Figure 4

(A) Immunohistochemistry and in situ hybridization were conducted to detect the expression of ERBB2 and miR-193a-5p in ESCC tumor samples. Scale bar, 100 μm. (B) In situ hybridization was conducted to examine the expression of miR-193a-5p in ESCC specimens. Scale bar, 500 μm (40×) and 50 μm (200×). (C) Real-time PCR showed the expression of miR-193a-5p in N-T paired specimens of twenty-eight patients with esophageal cancer; P = 0.001. (D) In unpaired normal and tumor tissues, miR-193a-5p was detected and analyzed using a Mann-Whitney test; P = 0.001. The cutoff value was 3.91, as determined by a ROC curve. (E) Kaplan-Meier plots of the overall survival of 131 ESCC patients stratified by miR-193a-5p expression level. (F) Spearman's correlation analysis showed the inversely correlation between ERBB2 and miR-193a-5p; P = 0.015, correlation coefficient = – 0.213.