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. 2016 May 23;7(26):39957–39969. doi: 10.18632/oncotarget.9554

Figure 5. RHAMM is crucial for the migration capacity of human lung adenocarcinoma cell lines.

Figure 5

(A) Confirmation of RHAMM knockdown in H1975 and H3255 cell lines. Western blot analysis of RHAMM and GAPDH (as a loading control) in the two human lung adenoma cell lines. (B and C) Inhibition of H1975 and H3255 migration with shRHAMM was determined using in vitro transwell migration chamber with a serum gradient (0% to 10%). Migrated cells on the bottom of the plate (B) or the bottom surface of the transwell inserts (C) were counted from 8 randomly picked fields under 10 × magnifications. Error bars represent s.e.m.