Figure 3. Combination of Nutlin-3/RG7388 with cisplatin increased stabilization of p53 and upregulation of its downstream targets, MDM2 and p21^WAF1 compared to cisplatin on its own.

Total levels of p53, p21^WAF1, MDM2 (4 hours) and BAX (8 hours) after the commencement of treatment with Nutlin-3 and RG7388 alone, and in combination with cisplatin at constant 1:1 ratios of 1X and 2X their respective GI₅₀ concentration analyzed by western blot in three wild-type TP53 ovarian cancer cell lines. Nutlin-3 and RG7388 led to little change in BAX compared to DMSO control and combination of Nutlin-3 with cisplatin at 1X GI₅₀ showed only a slight increase in the expression of BAX in A2780 and IGROV-1 cell lines compared to cisplatin on its own.