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. 2016 May 31;7(26):40314–40328. doi: 10.18632/oncotarget.9739

Figure 1. MZF1 facilitates the expression of MMP-14 in gastric cancer cells.

Figure 1

(A), scheme of potential binding sites of MZF1 and miR-337-3p within the MMP-14 promoter, locating at bases −98/−88 and −90/−71 relative to TSS. (B), western blot showing the expression of MZF1 and MMP-14 in normal gastric epithelial GES-1 cells and gastric cancer cell lines (AGS, SGC-7901, MKN-28, and MKN-45). (C and D), western blot and real-time quantitative RT-PCR indicating the expression of MZF1 and MMP-14 in AGS and SGC-7901 cells stably transfected with empty vector (mock) or MZF1. (E) nuclear run-on assay showing the nascent MMP-14 transcript levels in gastric cancer cells stably transfected with mock or MZF1. (F and G), western blot and real-time quantitative RT-PCR indicating the MZF1 and MMP-14 levels in MKN-45 and SGC-7901 cells stably transfected with scramble shRNA (sh-Scb) or MZF1 shRNA (sh-MZF1). (H), nuclear run-on assay showing the nascent MMP-14 transcript levels in gastric cancer cells transfected with sh-Scb or sh-MZF1. *P < 0.01 vs. GES-1, mock, or sh-Scb.