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. 2016 May 31;7(26):40314–40328. doi: 10.18632/oncotarget.9739

Figure 2. MZF1 increases the transcription of MMP-14 through direct binding to its promoter.

Figure 2

(A), scheme and sequence of the intact MZF1 binding site (WT) and its mutation (Mut) within the MMP-14 promoter-luciferase reporter vectors. (B), dual-luciferase assay showing the activity of MMP-14 promoter and its mutant in AGS and SGC-7901 cells stably transfected with empty vector (mock) or MZF1. (C), dual-luciferase assay indicating the activity of MMP-14 promoter and its mutant in MKN-45 and SGC-7901 cells stably transfected with scramble shRNA (sh-Scb) or MZF1 shRNA (sh-MZF1). (D), ChIP and qPCR assay (normalized to input DNA) showing the enrichment of MZF1 on the MMP-14 promoter in AGS, SGC-7901, and MKN-45 cells transfected with mock, MZF1, sh-Scb, or sh-MZF1. *P < 0.01 vs. mock, sh-Scb, or IgG.