Figure 1. ELISA of the selected phage clones toward Clec9a-CTLD protein.

10μg Clec9a-CTLD protein was coated into the ELISA plate at 4°C overnight. The next day, the plate was washed 3 times with TBS buffer containing 0.05% Tween 20 and blocked with 5% BSA buffer. 1 × 10⁷ phages were added for 2 hours at room temperature. The anti-M13 protein with HRP and 1×TMB buffer were used. Finally, 50 μL 1mM H₃PO₄ stop buffer was added and plate was read at 450nm. #: twice higher than control group (CTR, without phage). CTR phage: phage that do not have random peptide sequence inserted.