Figure 4. The effects of peptide WH on the cross-presentation of OVA_257-264 by FL-DCs in vitro.

FL-DCs were pulsed with different peptides (10μg/mL) or normal saline at 4°C for 1.5 h, and then washed 3 times with medium. Isolated lymph node CD8⁺ T cells from OT-1 mice were labeled with 2μM CFSE for 10min at room temperature, and washed 3 times with medium. The 1×10⁵ CFSE stained CD8⁺ T cells were incubated with different peptides pulsed 1×10⁴ FL-DCs for 72 h. A. The IFN-γ of the supernatant determined by ELISA. B. Proliferation of CD8⁺ T cells stimulated by peptide pulsed FL-DCs. C. The perforin and granzyme B (Grz B) mRNA expression fold change compared to the control peptide GA-OVA_257-264 group. Data are presented as means ± SEM, **P<0.01, ***P<0.001. Data are representative of three experiments.