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. 2016 May 26;7(26):40437–40450. doi: 10.18632/oncotarget.9624

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Copyright: © 2016 Yan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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100μg WH-OVA/OVA_257-264/WH/GA-OVA or normal saline (NS) was injected s.c. with CpG ODN 1826 (30μg) weekly for three times, then the splenocytes were isolated and re-stimulated by OVA_257-264 (10μg/mL) for 5 days. A. The scheme of the immunization and process. B. Supernatant IFN-γ determined by ELISA. C. The frequencies of IFN-γ released by OVA_257-264 specific CD8⁺ T cells analyzed by flow cytometry intracellular staining. The histogram showed the statistical results. D. The perforin and granzyme B mRNA expression fold change compared to the control peptide GA-OVA_257-264 group. Data are presented as means ± SEM (n=5), *P<0.05, ** P<0.01, *** P<0.001. Data are representative of two experiments.

100μg WH-OVA/OVA_257-264/WH/GA-OVA or normal saline (NS) was injected s.c. with CpG ODN 1826 (30μg) weekly for three times, then the splenocytes were isolated and re-stimulated by OVA_257-264 (10μg/mL) for 5 days. A. The scheme of the immunization and process. B. Supernatant IFN-γ determined by ELISA. C. The frequencies of IFN-γ released by OVA_257-264 specific CD8⁺ T cells analyzed by flow cytometry intracellular staining. The histogram showed the statistical results. D. The perforin and granzyme B mRNA expression fold change compared to the control peptide GA-OVA_257-264 group. Data are presented as means ± SEM (n=5), *P<0.05, ** P<0.01, *** P<0.001. Data are representative of two experiments.