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. 2016 Jun 3;7(26):40657–40673. doi: 10.18632/oncotarget.9813

Figure 5. miR-558 promotes the growth, invasion, and angiogenesis of NB cells through facilitating HIF-2α expression in vitro.

Figure 5

A. western blot indicating the expression of HIF-2α in SH-SY5Y and SK-N-SH cells stably transfected with empty vector (mock) or miR-558 precursor, and those co-transfected with sh-Scb, sh-AGO2, sh-eIF4E, or sh-HIF-2α. B. representation (left) and quantification (right) of colony formation assay showing the growth of SH-SY5Y and SK-N-SH cells stably transfected with mock or miR-558 precursor, and those co-transfected with sh-Scb, sh-AGO2, sh-eIF4E, or sh-HIF-2α. C. representation (left) and quantification (right) of matrigel invasion assay indicating the invasion capability of SH-SY5Y and SK-N-SH cells stably transfected with mock or miR-558 precursor, and those co-transfected with sh-Scb, sh-AGO2, sh-eIF4E, or sh-HIF-2α. D. representation (left) and quantification (right) of tube formation assay showing the angiogenic capability of NB cells stably transfected with mock or miR-558 precursor, and those co-transfected with sh-Scb, sh-AGO2, sh-eIF4E, or sh-HIF-2α. * P<0.01 vs. mock+sh-Scb.