Table 1. Read pairs assigned human (hg19) and mouse (mm10) by both the disambiguate and xenome algorithms.
The ’ambiguous’ column includes reads that aligned but could not be unambiguously disambiguated. The † symbol and the numbers in parentheses indicate false positive reads prior to applying the disambiguation algorithm on the raw alignments. TP denotes true positive and FP false positive.
| Tool | Material | Sample | Total
reads |
Mouse mm10 | Human hg19 | ambiguous |
|---|---|---|---|---|---|---|
| disambiguate | DNA | SRR1176814 (mouse) | 47312349 | 47197650 (99.76%) TP | 26157(0.06%) FP
(25638785 (54.19%)) † |
88542 (0.19%) |
| xenome | DNA | SRR1176814 (mouse) | 47312349 | 46889894 (99.11%) TP | 20031 (0.04%) FP | 339326 (0.72%) |
| disambiguate | DNA | SRR1528269 (human) | 77268164 | 11502 (0.01%) FP
(39686392 (51.36%)) † |
77102895 (99.79%) TP | 153767 (0.20%) |
| xenome | DNA | SRR1528269 (human) | 77268164 | 3291 (0.004%) FP | 76593625 (99.13%) TP | 521239 (0.67%) |
| disambiguate | RNA | SRR1930152 (mouse) | 24056144 | 23126086 (96.13%) TP | 80694 (0.34%) FP
(3005372 (12.49%)) † |
849364 (3.53%) |
| xenome | RNA | SRR1930152 (mouse) | 24056144 | 23071432 (95.91%) TP | 43294 (0.18%) FP | 625302 (2.60%) |
| disambiguate | RNA | SRR387400 (human) | 59653070 | 94289 (0.16%) FP
(6001230 (10.06%)) † |
49677937 (83.28%) TP | 9880844 (16.56%) |
| xenome | RNA | SRR387400 (human) | 59653070 | 83621 (0.14%) FP | 53851984 (90.28%) TP | 2043780 (3.43%) |