Figure 3.

ALDH1L1 promoter variants coupled with AAV serotypes 9, 8, and 5 direct transgene expression to nigral neurons. (a) Neuronal Luc expression mediated by ALDH11L1 promoters in the context of AAV9, AAV8, and AAV5 (represented by ALDH1L1(L) vector constructs). (b) Intensity of Luc immunoreactivity in the SNpc. (c) Total volume of transduction (mm³) within the SNpc, SNpr, and VTA. Bars represent mean ± SEM, n = 3, One-way analysis of variance (ANOVA), *P < 0.05, **P < 0.01. Scale bars for (a) 500 µm for low-power image and 100 µm for high-power inset. AAV, adeno-associated viral; ALDH1L1, aldehyde dehydrogenase family 1, member L1; Luc, luciferase; SEM, standard error of mean; SNpc, substantia nigra pars compacta; SNpr, SN pars reticulata; VTA, ventral tegmental area.