Skip to main content
. Author manuscript; available in PMC: 2017 Nov 17.
Published in final edited form as: Cell. 2016 Nov 10;167(5):1215–1228.e25. doi: 10.1016/j.cell.2016.10.028

Figure 5. Position of the Nup82-Nup84 complex assembly within the NPC.

Figure 5

(A) Fitting to the yeast NPC map. Two views of the optimized alignment of two S. cerevisiae Nup82-Nup84 complex assemblies into the S. cerevisiae NPC localization probability density map (transparent gray), together with a side view of the detailed alignment (Alber et al., 2007b); Nup85 (green), Nup133 (red), and two Nup82 units (blue and orange) are indicated. Scale bars, 100 Å.

(B) Comparison with the human NPC tomographic cryo-EM map (EMDB 2444) (Bui et al., 2013). Two views of the optimized alignment of two S. cerevisiae Nup82-Nup84 complex assemblies (pink and blue) into the human NPC map (CCC = 0.72). One suggested localization for the human Nup214/Nup88 complex is colored in yellow.

(C) Comparison with the mutant human NPC tomographic cryo-EM map (EMDB 3104) (von Appen et al., 2015), lacking an outer cytoplasmic Y-complex ring (CCC = 0.81). See also Figure S7.