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. 2016 Nov 30;11(11):e0167225. doi: 10.1371/journal.pone.0167225

Fig 8. Western blot analysis of sera from immunized mice.

Fig 8

5 groups of 3 mice (A, B, C) were orally immunized with recombinant spores of strains B. subtilis 168; 2-BTL20(CotC-M2eH-A-S-H), BTL21(CotB-M2eH-A-S-H), BTL22(CotZ-M2eH-A-S-H) or BTL25(CgeA-M2eH-A-S-H). A 15 μg of recombinant M2eH-A-S-H-GST was separated in polyacrylamide gel and further electrotransferred on nitrocellulose which was further incubated with sera from immunized mice as a primary antibodies. Blots were developed using anti-mouse alkaline phosphatase conjugates. Figure is a merge of 20 different single-lane blot strips.