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. 2016 Nov 30;11(11):e0167225. doi: 10.1371/journal.pone.0167225

Table 2. List of PCR oligonucleotides.

Name Sequence Restriction site
cotB-F GCGGATCCGGATGATTGAT BamHI
cotB-R GATGAATTCACGGATTAGGCC EcoRI
cotBlinker-F CGCGGATCCTCCTCCACCTTTCGCTGCTGCTTCTCCTCCACCGGATGATTGATCATCTGAAG BamHI
cotBlinker-R GCCTGTTAGGAATTCCGCTCCAATCTCTTTTTACAATAGAATATATGGAACCGAAAATCATGGCGATGTATGAACGGATTAGGCC EcoRI
cotC-F GGGGATCCGTAGTGTTTTTTATGC BamHI
cotC-R CAGAATTCTGTAGGATAAATCGTTTGG EcoRI
cotZ-F GCTTAGGATCCATGATGATGTGTACGATTG BamHI
cotZ-R CGTAGCGAATTCAGTTATCACTCTTGTCCTC EcoRI
cotZlinker-F CGCGGATCCTCCTCCACCTTTCGCTGCTGCTTCTCCTCCACCATGATGTGTACGATTGAT BamHI
cotZlinker-R CCGGAATTCGCAACCCTTATTTCTACAGCAACAAATACACTCGTAGCCATCCTAGTTATCACT EcoRI
cgeA-F CGGGGATCCTGAAAAGAACGTAAC BamHI
cgeA-R CAGCTTAGAATTCTTGAGAGTGAAACATGAG EcoRI
cgeAlinker-F CGCGGATCCTCCTCCACCTTTCGCTGCTGCTTCTCCTCCACCTGAAAAGAACGTAACGCTTTC BamHI
cgeAlinker-R CCGGAATTCAAGCAGAGCCTCTGTCATCATTTAAAAAGCACCCCAGCTTACAACACTTGA EcoRI
AmyA CGAGAAGCTATCACCGCCCAGC -
AmyS CCAATGAGGTTAAGAGTATTCC -