Table 2. Effect of TGFβ1 addition on expression of TGFβ signaling target genes in ADSCs from DSLD-Pasos and NA-Pasos.
| mRNA Abundance1 | Fold-change with TGFβ12 | Fold Difference3 | |||
|---|---|---|---|---|---|
| Genes | DSLD Paso | NA Paso | DSLD Paso | NA Paso | DSLD Paso:NA Paso |
| PTK2B | 0.15 | 0.23 | -4.13 | -14.78 | -1.53 |
| ATF3 | 5.41 | 5.47 | 8.07 | 1.65 | -1.01 |
| MAPK14 | 1.86 | 3.29 | -4.81 | -13.06 | -1.77 |
| ME2 | 3.47 | 8.86 | -1.57 | -2.77 | -2.55 |
| ACVRL1 | 0.05 | 0.14 | -5.80 | -8.50 | -2.80 |
| NFIB | 8.46 | 18.29 | -2.22 | -4.25 | -2.16 |
| EPHB2 | 2.83 | 6.39 | -3.16 | -5.47 | -2.26 |
| HMOX1 | 15.33 | 14.8 | -1.95 | -6.93 | 1.04 |
| SMAD6 | 1.92 | 2.91 | -2.55 | -5.50 | -1.52 |
| FOS | 8.49 | 3.21 | 1.94 | -4.33 | 2.64 |
| GLI2 | 0.25 | 0.58 | -1.84 | -2.53 | -2.32 |
| STC2 | 25.12 | 41.5 | 4.45 | 2.39 | -1.65 |
| ID2 | 19.91 | 23.66 | -2.35 | -6.02 | -1.19 |
| PPARA | 1.07 | 2.22 | -2.63 | -3.84 | -2.07 |
| ENG | 22.4 | 42.31 | -1.78 | -2.84 | -1.89 |
| CREBBP | 5.47 | 8.82 | -2.28 | -4.20 | -1.61 |
| NFKBIA | 6.41 | 9.02 | -1.61 | -3.39 | -1.41 |
| BRD2 | 24.8 | 33.3 | -1.28 | -2.82 | -1.34 |
| TGFBR2 | 12.9 | 24.11 | -2.86 | -4.46 | -1.87 |
| MMP2 | 170.2 | 234.2 | -1.84 | -3.86 | -1.38 |
| CDKN1B | 8.37 | 10.3 | -1.69 | -3.93 | -1.23 |
| EP300 | 4.39 | 8.16 | -2.06 | -3.15 | -1.86 |
| FURIN | 1.39 | 2.28 | 1.32 | -1.28 | -1.64 |
| MBD1 | 2.36 | 5.49 | -1.61 | -1.88 | -2.33 |
| IFRD1 | 12.2 | 19.18 | 1.70 | 1.04 | -1.57 |
| SMAD3 | 8.64 | 7.47 | -2.54 | -6.89 | 1.16 |
| BCL2L1 | 4.35 | 4.63 | -1.33 | -2.82 | -1.06 |
| MAPK8 | 4.41 | 7.3 | 1.00 | -1.32 | -1.66 |
| SMAD1 | 7.13 | 13.29 | -1.22 | -1.40 | -1.86 |
| RHOA | 142.2 | 204.6 | 1.03 | -1.43 | -1.44 |
| BACH1 | 6.56 | 9.67 | -1.50 | -2.08 | -1.47 |
| MYC | 19.23 | 48.95 | -1.72 | -1.33 | -2.55 |
| PAI-1 | 648.1 | 869.8 | 1.84 | 1.27 | -1.34 |
| ID3 | 239.3 | 292.4 | 1.12 | -1.42 | -1.22 |
| SNAI1 | 67.9 | 50.54 | 1.34 | -1.73 | 1.34 |
1Mean abundance data for NA-Pasos (n = 5) and DSLD-Pasos (n = 6) (with TGFβ treatment) is provided (see S1 Table for animal details).
2Fold-change relative to values in Table 1.
3 Fold difference in transcript abundance in TGFβ1-treated cultures (columns 1 and 2). Genes are listed in the same order as in Table 1. Calculation of statistical significance and abundance values are given in the Methods Section. Fold change with TGFβ, for all genes in the array is shown on heat map (Fig 2A and 2B).