Table 3. Effect of LY2109761 on TGFβ1 induced changes in transcript abundance of TGFβ1 signaling target genes in DSLD-Pasos and NA-Pasos.
| Genes | mRNA Abundance1 | Fold Change 2 | Fold Difference3 | ||
|---|---|---|---|---|---|
| (TGFβ1+LY2109761) | (TGFβ1+LY2109761:TGFβ1) | ||||
| DSLD Paso | NA Paso | DSLD Paso | NA Paso | DSLD Paso:NA Paso | |
| PTK2B | 2.66 | 3.29 | 17.76 | 14.31 | 1.23 |
| ATF3 | 0.79 | 3.67 | -6.88 | -1.49 | 4.66 |
| MAPK14 | 29.85 | 46.40 | 16.05 | 14.10 | 1.55 |
| ME2 | 18.91 | 21.03 | 5.45 | 2.37 | 1.11 |
| ACVRL1 | 1.15 | 2.03 | 22.93 | 14.52 | 1.77 |
| NFIB | 36.18 | 75.06 | 4.28 | 4.10 | 2.07 |
| EPHB2 | 31.30 | 29.53 | 11.06 | 4.62 | -1.06 |
| HMOX1 | 85.41 | 102.93 | 5.57 | 6.95 | 1.21 |
| SMAD6 | 15.24 | 11.82 | 7.94 | 4.06 | -1.29 |
| FOS | 10.70 | 23.67 | 1.26 | 7.37 | 2.21 |
| GLI2 | 0.84 | 1.27 | 3.35 | 2.19 | 1.52 |
| STC2 | 7.35 | 13.77 | -3.42 | -3.01 | 1.88 |
| ID2 | 119.71 | 98.05 | 6.01 | 4.14 | -1.22 |
| PPARA | 5.04 | 7.42 | 4.71 | 3.34 | 1.47 |
| ENG | 49.41 | 55.38 | 2.21 | 1.31 | 1.12 |
| CREBBP | 23.25 | 29.43 | 4.25 | 3.34 | 1.27 |
| NFKBIA | 23.63 | 24.23 | 3.69 | 2.69 | 1.02 |
| BRD2 | 54.19 | 73.51 | 2.19 | 2.21 | 1.36 |
| TGFBR2 | 98.17 | 160.95 | 7.61 | 6.68 | 1.64 |
| MMP2 | 544.85 | 657.55 | 3.20 | 2.81 | 1.21 |
| CDKN1B | 27.67 | 33.76 | 3.31 | 3.28 | 1.22 |
| EP300 | 14.32 | 19.97 | 3.26 | 2.45 | 1.39 |
| FURIN | 1.33 | 1.53 | -1.05 | -1.49 | 1.15 |
| MBD1 | 8.91 | 9.47 | 3.78 | 1.72 | 1.06 |
| IFRD1 | 9.83 | 12.16 | -1.24 | -1.58 | 1.24 |
| SMAD3 | 35.19 | 45.88 | 4.07 | 6.14 | 1.30 |
| BCL2L1 | 12.89 | 11.29 | 2.96 | 2.44 | -1.14 |
| MAPK8 | 7.96 | 8.60 | 1.81 | 1.18 | 1.08 |
| SMAD1 | 16.97 | 18.67 | 2.38 | 1.40 | 1.10 |
| RHOA | 302.47 | 282.66 | 2.13 | 1.38 | -1.07 |
| BACH1 | 14.59 | 20.28 | 2.22 | 2.10 | 1.39 |
| MYC | 32.81 | 63.36 | 1.71 | 1.29 | 1.93 |
| PAI-1 | 389.13 | 447.97 | -1.67 | -1.94 | 1.15 |
| ID3 | 251.33 | 285.67 | 1.05 | -1.02 | 1.14 |
| SNAI1 | 37.29 | 40.14 | -1.82 | -1.26 | 1.08 |
1Mean abundance data for NA-Pasos (n = 5) and DSLD-Pasos (n = 6) is provided (see S1 Table for animal details).
2 Fold difference in transcript abundance in TGFβ1-treated cultures in the presence and absence of LY2109761.
3Fold difference in transcript abundance in TGFβ1 +LY2109761 treated DSLD-Paso vs. TGFβ1 +LY2109761 treated NA-Paso cultures. Genes are listed in the same order as in Table 1