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. 2016 Nov 30;11(11):e0166969. doi: 10.1371/journal.pone.0166969

Table 3. Parentage of hybrid varieties with their organellar genotypes and the LOD scores used for error checking of DNA markers.

# Hybrid variety Seed parent Pollen parent LOD 2) RCI 3) SPP 4)
ID Variety name CT1) ID Variety name CT 1) ID Variety name CT1) score Seed Pollen
1 B001 Akemi C12 B031 Kiyomi C12 B056 Seminole C04 91.3 172.3 51.8 48.1
2 B003 Aki Tangor C12 A125 Satsuma C12 A162 Sweet orange C07 78.8 162.3 51.3 49.2
3 B005 Amaka C12 B031 Kiyomi C12 B014 Encore C12 95.9 169.0 47.5 55.3
4 B006 Ariake C07 A162 Sweet orange C07 A009 Clementine C12 108.0 185.3 49.2 50.9
5 B007 Asumi C12 B077 Okitsu-46 C12 B021 Harumi C12 99.5 171.8 49.3 53.7
6 B008 Aurastar C04 B072 H/FD-1 C04 A004 Banpeiyu C04 5) 5) 36.4 34.5
7 B009 Awa Orange C05 A036 Hyuganatsu C05 A162 Sweet orange C07 72.9 175.2 46.8 45.4
8 B010 Benibae C12 B073 HF9 C12 B014 Encore C12 100.9 161.1 54.3 55.5
9 B011 Benimadoka C04 A088 Mato buntan C04 A032 Hirado buntan C04 210.0 256.9 45.0 44.9
10 B014 Encore C12 A054 King C12 A201 Willowleaf mandarin C12 102.5 167.2 53.1 58.9
11 B015 Fairchild C12 A009 Clementine C12 B045 Orland C04 84.0 156.2 56.3 50.1
12 B017 Harehime C12 B070 E-647 C12 A125 Satsuma C12 103.3 179.5 49.6 46.4
13 B018 Hareyaka C12 B014 Encore C12 A107 Ponkan C12 104.0 163.9 56.7 55.1
14 B019 Haruhi C12 B077 Okitsu-46 C12 B009 Awa Orange C05 97.5 179.3 49.8 49.3
15 B021 Harumi C12 B031 Kiyomi C12 A107 Ponkan C12 94.6 172.0 49.5 51.9
16 B022 Hayaka C12 A125 Satsuma C12 A107 Ponkan C12 91.8 169.1 48.8 51.7
17 B023 Hayasaki C04 A088 Mato buntan C04 A032 Hirado buntan C04 209.5 257.1 44.4 44.2
18 B024 Himekoharu C12 B031 Kiyomi C12 A101 Oogonkan C05 81.4 171.5 49.0 47.0
19 B026 Honey C12 A054 King C12 A201 Willowleaf mandarin C12 114.5 181.6 52.9 56.0
20 B027 Kanpei C12 B039 Nishinokaori C12 A107 Ponkan C12 98.4 167.7 51.4 48.7
21 B028 Kara mandarin C12 A125 Satsuma C12 A054 King C12 77.2 148.8 53.9 54.0
22 B030 Kinnow mandarin C12 A054 King C12 A201 Willowleaf mandarin C12 104.2 171.6 52.3 57.5
23 B031 Kiyomi C12 A125 Satsuma C12 A162 Sweet orange C07 81.4 169.2 47.3 48.4
24 B033 Lee C12 A009 Clementine C12 B045 Orland C04 103.9 178.9 53.7 48.1
25 B034 May Pomelo C04 A028 Hassaku C04 A032 Hirado buntan C04 139.7 216.0 41.9 50.2
26 B035 Mihaya C12 B066 Tsunonozomi C12 B076 No.1408 C12 98.7 160.1 53.9 56.2
27 B036 Mihocore C12 A125 Satsuma C12 B014 Encore C12 97.5 163.9 53.9 56.2
28 B037 Mineola C04 A024 Grapefruit C04 A016 Dancy tangerine C12 90.2 164.0 47.5 52.1
29 B038 Nankou C12 A125 Satsuma C12 A009 Clementine C12 104.8 175.7 49.7 48.9
30 B039 Nishinokaori C12 B031 Kiyomi C12 A162 Sweet orange C07 94.8 186.5 47.9 44.6
31 B040 Nou 5 gou C12 B033 Lee C12 A059 Kishu C12 113.9 175.5 53.0 54.0
32 B041 Nou 6 gou C12 A054 King C12 A059 Kishu C12 106.3 174.4 53.6 53.0
33 B042 Nou 7 gou C04 B072 H/FD-1 C04 A004 Banpeiyu C04 5) 5) 37.7 38.8
34 B043 Nova C12 A009 Clementine C12 B045 Orland C04 119.1 193.3 51.2 46.3
35 B045 Orland C04 A024 Grapefruit C04 A016 Dancy tangerine C12 101.1 174.0 46.5 49.9
36 B047 Osceola C12 A009 Clementine C12 B045 Orland C04 100.5 176.9 53.9 48.7
37 B048 Page C04 B037 Mineola C04 A009 Clementine C12 110.7 180.3 49.3 53.5
38 B051 Robinson C12 A009 Clementine C12 B045 Orland C04 104.2 180.5 54.5 47.6
39 B052 Saga Mandarin C12 A125 Satsuma C12 B015 Fairchild C12 93.8 166.1 52.7 50.7
40 B054 Seihou C12 B031 Kiyomi C12 B037 Mineola C04 98.0 180.7 50.3 49.4
41 B055 Seinannohikari C12 B071 EnOw21 C12 B069 Youkou C12 92.7 160.1 54.5 52.7
42 B056 Seminole C04 A024 Grapefruit C04 A016 Dancy tangerine C12 93.3 171.6 46.3 51.1
43 B057 Setomi C12 B031 Kiyomi C12 A107 Ponkan C12 107.5 185.5 47.7 48.4
44 B058 Shiranuhi C12 B031 Kiyomi C12 A107 Ponkan C12 82.4 161.9 52.8 49.5
45 B059 Southern Yellow C05 B063 Tanikawa Buntan C05 A059 Kishu C12 104.8 185.1 46.1 42.0
46 B060 Summer Fresh C04 A028 Hassaku C04 A098 Natsudaidai C04 94.0 191.0 45.6 42.9
47 B061 Sweet Spring C12 A125 Satsuma C12 A028 Hassaku C04 69.3 155.5 52.3 48.1
48 B062 Tamami C12 B031 Kiyomi C12 B067 Willking C12 109.6 174.5 49.9 53.9
49 B064 Tsunokagayaki C12 B074 KyOw14 C12 B014 Encore C12 94.7 164.2 52.5 52.3
50 B065 Tsunokaori C12 B031 Kiyomi C12 A125 Satsuma C12 84.9 159.5 52.0 45.9
51 B066 Tsunonozomi C12 B031 Kiyomi C12 B014 Encore C12 94.1 165.4 50.4 53.3
52 B068 Yellow Pummelo C04 A028 Hassaku C04 A032 Hirado buntan C04 108.5 183.5 47.5 52.6
53 B069 Youkou C12 B031 Kiyomi C12 A107 Ponkan C12 77.2 159.1 50.3 52.4
54 B070 E-647 C12 B031 Kiyomi C12 B047 Osceola C12 111.8 183.7 49.3 49.9
55 B071 EnOw21 C12 B014 Encore C12 A125 Satsuma C12 97.2 165.5 52.5 52.2
56 B073 HF9 C12 A125 Satsuma C12 A162 Sweet orange C07 72.9 165.8 48.4 46.3
57 B074 KyOw14 C12 B031 Kiyomi C12 A125 Satsuma C12 91.7 170.1 51.3 50.6
58 B075 KyOw21 C12 B031 Kiyomi C12 A125 Satsuma C12 96.9 174.3 51.4 48.8
59 B077 Okitsu-46 C12 B061 Sweet Spring C12 A162 Sweet orange C07 76.9 173.0 46.8 47.0

1) Category of organellar genotype (see Table 9).

2) LOD: See the section ‘Stochastic evaluation of inferred parentage’.

3) RCI: required cross trial index obtained by Eq 7.

4) SPP: Single parent–offspring probability for seed parent or pollen parent obtained by Eq 8.

5) Score or value was indeterminate due to lack of allele in H/FD-1.

All DNA markers were also evaluated for error with 64 selected strains.