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. 2016 Nov 30;12(11):e1006024. doi: 10.1371/journal.ppat.1006024

Fig 4. Confocal microscopy of biofilms stained with PIA/PNAG-specific antibodies.

Fig 4

Non-mucoid strain (A) and mucoid (B) S. aureus strains (strains 8.1 and 8.2) were grown overnight in TSB. After washing, adherent bacteria were stained using SYTO 9 (green). PIA/PNAG was detected using ALEXA-568 labeled wheat germ agglutinin (red), which is a lectin binding to the N-acetylglucosaminyl backbone of PIA/PNAG. Zoom-in shows PIA/PNAG-embedded bacteria in the mucoid strain (B), while hardly any PIA/PNAG is detected with the non-mucoid isolate. White bar = 10 μm.