Skip to main content
. Author manuscript; available in PMC: 2016 Nov 30.
Published in final edited form as: Osteoarthritis Cartilage. 2007 Sep 27;16(4):458–469. doi: 10.1016/j.joca.2007.08.006

Fig. 5.

Fig. 5

Comparison of the tissue changes to the medial joint surfaces. Sagittal in vivo micro-CT image of the ACLX (a) and LEFT (b) rodent hind limbs 5 months post-surgery. Note the subchondral plate thickening and loss of mature trabecular bone pattern (a) vs the LEFT limb (b). Joints were fixed, decalcified, and processed for histological staining with Safranin-O and Fast Green. The histological sections were manually registered to the boxed areas of the micro-CT images. Note the delamination of cartilage within the ACLX joint (c), a small remaining tissue fragment (arrow) containing a cluster of chondrocytes. The subchondral bone was invaded at several points (c – vertical arrowhead); while the underlying bony trabeculae were surrounded by fibrous tissue (c – horizontal arrowheads). The cartilage and bone tissue of LEFT limb (d) appeared normal with a consistent, uninterrupted joint surface (arrowheads) and evenly distributed chondrocytes. Scale bar in (c) and (d) = 0.5 mm.