Table 2. .
Real-time RT-PCR primer sequences
| Target | Forward primer | Reverse primer | Tm* (°C) | Product size (bp) |
|---|---|---|---|---|
| RohA | 5'-cgcttttgggtacatggagt-3' | 5'-caagacaaggcacccagatt-3' | 60.0 | 124 |
| ROCK | 5'-cttttgccaacagtccttggg-3' | 5'-acaagatctccaccaggcatg-3' | 60.0 | 100 |
| PIK3CA | 5'-ctctgcaaaaaggccactgt-3' | 5'-gccgtaaatcatccccattt-3' | 60.4 | 107 |
| AKT1 | 5'-gcaccttccatgtggagact-3' | 5'-tgagttgtcactgggtgagc-3' | 60.1 | 130 |
| mTOR | 5'-ttaatggaggcccaagagtg-3' | 5'-gctttgagattcgtcggaac-3' | 60.1 | 109 |
| GAPDH | 5'-ggaaggtgaaggtcggagtca-3' | 5'-gtcattgatggcaacaatatccact-3' | 60.0 | 101 |
*Tm: melting temperature