Zinc finger protein 407 (Zfp407) mRNA overexpression in ZFP-TG mice. A: transgene construct with mouse Zfp407 cDNA tagged with Myc and DDK. B–D: endogenous (B), transgenic (C), and total Zfp407 mRNA expression (D) were measured by quantitative PCR and normalized to levels of Arbp in wild-type (WT) and ZFP-TG (TG) mice (n = 4–10 mice/group). *P < 0.05; **P < 0.01; ***P < 0.001. CMV, cytomegalovirus promoter; polyA, human growth hormone polyA signal; SQ, subcutaneous.