Table 2.
Metabolic trait data for CD- or HFD-fed 135-day-old ZFP-TG and WT male mice
| CD |
HFD |
3-Way ANOVA (P Value) |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Trait | WT | SE | n | ZFP-TG | SE | n | WT | SE | n | ZFP-TG | SE | n | Genotype | Sex | Diet | Interactions |
| Body Weight, g | 29.09 | 0.50 | 18 | 25.80 | 0.53 | 14 | 38.63 | 1.69 | 20 | 32.89 | 1.41 | 16 | 3.81e-06 | <2.2e-16 | 9.08e-13 | Sex/diet, P = 0.005 |
| Total weight gained, g | 8.53 | 0.51 | 16 | 7.01 | 0.64 | 11 | 18.36 | 1.42 | 20 | 14.66 | 1.08 | 16 | 1.80e-03 | 4.11e-14 | 3.72e-16 | Sex/diet, P < 0.001 |
| %Weight gained | 41.87 | 2.87 | 16 | 39.83 | 5.02 | 11 | 89.78 | 6.13 | 20 | 82.47 | 7.03 | 16 | NS | 1.10e-08 | 1.73e-15 | Sex/diet, P = 0.006 |
| %Fat mass | 17.91 | 0.92 | 14 | 17.62 | 1.08 | 10 | 26.20 | 1.56 | 20 | 23.27 | 1.75 | 16 | NS | 0.001773 | 3.62e-07 | NS |
| %Lean mass | 82.09 | 0.92 | 14 | 82.38 | 1.08 | 10 | 73.80 | 1.56 | 20 | 76.73 | 1.75 | 16 | NS | 0.01034 | 6.13e-08 | NS |
| Perigonadal fat mass, g | 0.27 | 0.04 | 14 | 0.24 | 0.05 | 10 | 0.95 | 0.12 | 20 | 0.71 | 0.13 | 14 | 0.034161 | 3.77e-08 | 7.08e-08 | Sex/diet, P = 0.003 |
| Inguinal fat mass, g | 0.16 | 0.03 | 14 | 0.15 | 0.02 | 10 | 0.60 | 0.08 | 20 | 0.43 | 0.07 | 14 | 0.032657 | 5.23e-06 | 8.42e-09 | Sex/diet, P = 0.008 |
| Length, mm | 98.94 | 0.62 | 19 | 95.93 | 0.89 | 14 | 102.00 | 0.57 | 16 | 100.86 | 1.14 | 14 | 0.005326 | 0.002286 | 5.20e-08 | Genotype/diet, P = 0.03 |
| Body mass index, kg/m2 | 3.00 | 0.63 | 18 | 2.86 | 0.84 | 16 | 3.71 | 1.40 | 20 | 3.20 | 0.91 | 15 | 5.87e-06 | <2.2e-16 | 4.46e-09 | Genotype/diet, P = 0.03 |
| Fasting glucose, mg/dl | 96.71 | 4.98 | 18 | 84.29 | 5.45 | 14 | 145.85 | 8.51 | 20 | 132.69 | 8.55 | 16 | 2.25e-02 | 0.01764 | 1.21e-09 | Genotype/diet, P = 0.03 |
| Fasting insulin, ng/ml | 0.34 | 0.06 | 8 | 0.23 | 0.06 | 7 | 0.44 | 0.11 | 10 | 0.35 | 0.07 | 9 | NS | NS | NS | NS |
| HOMA-IR | 2.10 | 0.41 | 8 | 1.54 | 0.51 | 7 | 3.69 | 0.75 | 10 | 3.44 | 0.88 | 9 | NS | 0.01524 | 0.03014 | NS |
| GTT AUC | 38270 | 2719 | 8 | 32,610 | 1851 | 7 | 43551 | 2636 | 10 | 34,917 | 2,343 | 9 | 0.0008729 | 8.31e-13 | 0.0078087 | NS |
| Plasma β-hydroxybutyrate, mg/dl | 21.01 | 0.97 | 9 | 17.21 | 1.69 | 9 | 13.56 | 1.57 | 10 | 12.50 | 1.52 | 9 | NS | 0.011259 | 0.002899 | NS |
| Plasma cholesterol, mg/dl | 121.56 | 5.58 | 9 | 117.22 | 10.00 | 9 | 145.70 | 14.37 | 10 | 122.33 | 10.70 | 9 | 0.03804 | 2.24e-05 | NS | NS |
| Plasma nonesterified fatty acids, mg/dl | 138.00 | 9.99 | 9 | 113.44 | 10.19 | 9 | 117.40 | 11.84 | 10 | 97.33 | 7.37 | 9 | NS | NS | NS | NS |
| Plasma triglycerides, mg/dl | 1.07 | 0.03 | 9 | 1.11 | 0.06 | 9 | 0.90 | 0.05 | 10 | 1.09 | 0.12 | 8 | 0.008887 | 2.06e-05 | 0.024818 | NS |
CD, control diet; HFD, high-fat diet; GTT AUC, glucose tolerance test area under the curve.