Table 2.
Gene ID | Product Size, bp | Primer | Location | Sequence 5′–3′ |
---|---|---|---|---|
Mouse PAI-1 (NM_008871.1) | 219 | Forward | 1122–1141 | AGT CTT TCC GAC CAA GAG CA |
Reverse | 1311–1340 | ATC ACT TGC CCC ATG AAG AG | ||
Mouse FN (NM_010233.1) | 226 | Forward | 7790–7807 | CCG TGG GAT GTT TGA GAC |
Reverse | 7998–8015 | GGC AAA AGA AAG CAG AGG | ||
Mouse α1(I) collagen (NM_007742.3) | 269 | Forward | 3765–3784 | CAC CCT CAA GAG CCT GAG TC |
Reverse | 4014–4033 | GCT TCT TTT CCT TGG GGT TC | ||
Mouse α1(IV) collagen (NM_009931.1) | 252 | Forward | 4629–4628 | ATG CCC TTT CTC TTC TGC AA |
Reverse | 4861–4880 | GAA GGA ATA GCC GAT CCA CA | ||
Mouse TGF-β1 (NM_011577.1) | 292 | Forward | 1447–1466 | TGA GTG GCT GTC TTT TGA CG |
Reverse | 1719–1738 | TCT CTG TGG AGC TGA AGC AA | ||
Mouse β-actin (NM_009606.1) | 295 | Forward | 857–876 | GCT CTT TTC CAG CCT TCC TT |
Reverse | 1132–1151 | TGA TCC ACA TCT GCT GGA AG |
FN, fibronectin; TGF, transforming growth factor.