Table 1.
A summary of operation, assay, and postprocessing of a cohort of embryos for day 3 analysis and a cohort of embryos for day 8 analysis
| Cohort 1: | Day 1 (HH stage 8–9) |
Day 8 (HH stage 34–35) |
Postprocessing | ||
|---|---|---|---|---|---|
| Operation | Assay | Purpose | Sample size (sham, experimental) | ||
| Sham/ablation | OCT volume imaging ex vivo | Great vessels | n = 8, 18 | Morphology analysis, diameter quantification | |
| Valves | n = 9, 24 | Segmentation, morphology analysis, quantification of leaflets | |||
| Cohort 2: | Day 1 (HH stage 8–9) |
Day 3 (HH stage 19–20) |
Postprocessing | ||
|---|---|---|---|---|---|
| Operation | Assay | Purpose | Sample size (sham, experimental) | ||
| Sham/Ablation | Pulsed Doppler OCT in vivo | Flow | n = 8, 26 | Waveform analysis and retrograde flow quantification | |
| OCT volume imaging in vivo | Body flexure | n = 8, 26 | Combining images | ||
| OCT volume imaging ex vivo | Cardiac cushions | n = 5, 10 | Morphology analysis and quantification | ||
HH, Hamburger and Hamilton; OCT, optical coherence tomography.