Fig. 2.
N6-CPA pretreatment decreases phospho-JNK signaling following endotoxin stimulation in MH-S cells. A: MH-S cells were pretreated with 10 μM N6-CPA for 1 h and then stimulated with LPS (100 ng/ml) for the indicated time points, pictured blots are representative of 3 independent experiments. Phosphorylation of the terminal MAPKs JNK (B), p38 (C), ERK (p42; D), and ERK (p44; E) were assessed by semiquantitative densitometry. Statistical analysis was performed using two-way ANOVA test with Sidak's correction for multiple comparisons. **P < 0.01.