Table 2.
Undetected lipids in conditioned medium of IL-1β-activated primary HLFs
| Eicosanoids | Docosanoids | Octadecanoids |
|---|---|---|
| Prostaglandins | 7(S)-maresin1 | 9,10-diHOME |
| 8-iso PGF2α | PD1 | 12(13)-epOME |
| 2,3-dinor PGE1 | 8-oxoRvD1 | 13(S)-HOTrE |
| Bicyclo PGE2 | 17-oxoRvD1 | 9-oxoOTrE |
| Bicyclo PGE1 | RvD2 | 13-oxoODE |
| 15-keto PGE2 | RvD3 | 9-oxoODE |
| PGD3 | RvD4 | |
| PGF3α | RvD5 | |
| 15-keto PGE1 | 10S,17S-diHDoHE | |
| D17-PGE1 | 4,17-diHDoHE | |
| PGD2 | 7,17-diHDoPE | |
| 11b-PGF2α | 19,20-diHDoPE | |
| 13,14dh-15k-PGE1 | 7(8)-epDPE | |
| 13,14dh-PGE1 | 10(11)-epDPE | |
| 6-keto PGE1 | 13(14)-epDPE | |
| Thromboxanes | 16(17)-epDPE | |
| 11dh-2,3-dinor TXB2 | 11-HDoHE | |
| 2,3-dinor TXB2 | 7-HDoHE | |
| 11dh-TXB3 | 10-HDoHE | |
| 11dh-TXB2 | 14-HDoHE | |
| Leukotrienes | 8-HDoHE | |
| LTB5 | 17-HDoHE | |
| LTB4 | 16-HDoHE | |
| 18-carboxy dinor LTB4 | ||
| 20-hydroxy LTB4 | ||
| Other eicosanoids | ||
| 11(R)-HEDE | ||
| 15(S)-HEDE | ||
| 15-oxo-EDE | ||
| 5,6-epETrE | ||
| Hydroxy/hydroperoxy-eicosatetraenoic acids | ||
| 5(S),6(S)-diHETE | ||
| 5(S),12(S)-diHETE | ||
| 5(S),15(S)-diHETE | ||
| 5,6-diHETE (EPA) | ||
| 8,9-epETE | ||
| 11,12-epETE | ||
| 9-HETE | ||
| 12-HETE | ||
| 15-HETE | ||
| 20-HETE | ||
| 5-oxoETE | ||
| Lipoxins | ||
| LXA4 | ||
| 15-epi LXA4 | ||
| 15-oxoLXA4 | ||
| LXA5 | ||
| LXB4 | ||
| Hydroxy/hydroperoxy-eicosatrienoic acids | ||
| 5,6-diHETrE | ||
| 8,9-diHETrE | ||
| 11,12-diHETrE | ||
| 5(S)-HETrE | ||
| 12(S)-HHTrE | ||
| Hydroxy/hydroperoxy-eicosapentaenoic acids | ||
| RvE1 | ||
| RvE3 | ||
| 5-HEPE | ||
| 9-HEPE | ||
| 8-HEPE | ||
| 11-HEPE | ||
| 12-HEPE | ||
| 18-HEPE |
Primary HLFs were treated with IL-1β (0.1 ng/ml) in the presence or absence of celecoxib (10 μM) for 72 h, and conditioned medium was subjected to targeted lipidomics analysis using quantitative LC-MS. Blank medium (i.e., serum-free phenol red-free MEM, 3 replicates) was submitted and analyzed to detect background levels of lipids. Lipids listed were nondetectable in conditioned media samples above background levels in blank medium. Background subtraction was performed on each conditioned medium sample by subtraction of lipid levels of the blanks (mean + 2SD) from each sample measurement.