Skip to main content
. 2016 Sep 9;311(5):L913–L923. doi: 10.1152/ajplung.00276.2016

Fig. 4.

Fig. 4.

ACR and related thiol-reactive electrophiles inhibit allergen-induced activation of EGFR, ERK1/2, and STAT3. A and B: HBE1 cells were pretreated with ACR (30 min) prior to stimulation with HDM or ATP for 15 min and pEGFR (Y1068), pERK1/2, or pSTAT3, as well as their unphosphorylated forms, were analyzed by Western blot. A: representative Western blots. B: densitometry analysis of pEGFR (Y1068), pERK1/2, or pSTAT3, normalized to unphosphorylated proteins, from 3 experiments in duplicate. *P < 0.05. C and D: HBE1 cells were pretreated with ACR, CUR, CINN, or SFN (30 min) prior to stimulation with ATP for 15 min and pEGFR (Y1068), pERK1/2, or pSTAT3 was determined by Western blot. C: representative Western blots. D: densitometry analysis of pEGFR (Y1068), pERK1/2, or pSTAT3. *P < 0.05.