Fig. 6.

ACR and other thiol-reactive electrophiles inhibit allergen-induced H₂O₂ production by HBE cells. A: HBE1 cells were pretreated with ACR (30 min) prior to stimulation with HDM, ALT, or ATP, and production of extracellular H₂O₂ in conditioned medium was measured after 15 min as described in materials and methods. B: dose-dependent inhibition of ATP-induced H₂O₂ production by ACR, SFN, or CINN. Data were expressed relative to ATP-stimulated H₂O₂ production in the absence of electrophiles and corrected for basal H₂O₂ production in the absence of ATP. C: inhibition of ionomycin-stimulated H₂O₂ production by ACR, SFN, or CINN (30 μM). *P < 0.05.