Fig. 7.

Western blot analysis of liver samples. A: Western blot analysis of 8 healthy and 3 fatty female liver samples show large changes in metabolic enzyme levels in Tg^+/+ animals compared with healthy mice. B: quantitation of changes in protein levels involved in lipid metabolism in bloated mice were significantly different. Values are means ± SE. *P < 0.05, **P < 0.005, and ***P < 0.0005 compared with nonbloated mice (n = 6). Abbreviations of enzymes probed: ACC1, acetyl-CoA carboxylase (catalyzes the production of malonyl-CoA); ACSL1, acyl CoA synthetase 1, also known as long-chain fatty acid CoA ligase 1 (converts long-chain fatty acids into fatty acyl CoA esters); ATP CL, ATP citrate lyase; beclin-1, ATG6 autophagy related gene; FABP1, fatty acid binding protein 1 (normally found in liver); FABP4, fatty acid binding protein 4 also known as aP2 (adipocyte protein 2; normally found in adipocyte tissue of obese mice); FAS, fatty acid synthase; Hsp90, heat shock protein 90 (aids in protein degradation and serves as a chaperone protein for proper protein folding); LC3-I and LC3-II (ATG8-II; autophagy-related genes); PFKL, liver phosphofructokinase; PKM1, isoenzyme of pyruvate kinase.