Table 1.
Mice homozygous for transgene (Tg+/+) exhibit the bloated phenotype
| Genotype | Total No. Mice | No. Bloated | %Bloated |
|---|---|---|---|
| Cacnb2+/+/Tg−/− | 10 | 0 | 0 |
| Cacnb2+/+/Tg+/− | 1 | 0 | 0 |
| Cacnb2+/+/Tg+/+ | 5 | 5 | 100 |
| Cacnb2−/−/Tg+/− | 4 | 0 | 0 |
| Cacnb2−/−/Tg+/+ | 4 | 4 | 100 |
| Cacnb2+/−/Tg+/− | 6 | 0 | 0 |
| Cacnb2+/−/Tg+/+ | 5 | 5 | 100 |
Mice from the UMMS colony were genotyped for transgene copy number. Quantitative PCR for DNA revealed that lesions were limited to mice bearing two copies of the transgene and were independent of native Cacnb2 genomic copy number.