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Decreased expression of SM contractile and regulatory protein in rat IAS. SMCs isolated from 6-mo-old vs. 26-mo-old rat IAS SMCs (A) were stained with the indicated antibodies. The images (taken on a Carl Zeiss LSM 510 UV META inverted confocal microscope) (A) compare the expression of the proteins between 6- and 26-mo-old rats (bar = 20 μm). B and C: significant decrease in immunofluorescence intensities for different proteins examined (*P < 0.05; n = 4).
