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. 2016 Sep 29;311(5):G954–G963. doi: 10.1152/ajpgi.00239.2016

Fig. 10.

Fig. 10.

Signaling pathway of SST regulation on NHE8 expression in TNF-α-treated Caco-2 cells. Cells were preincubated with TNF-α for 18 h and then coincubated with 1 μM SST, 1 μM seglitide, and 500 nM L-817,818 for 1 h. Total protein was prepared from cells and used for Western blot analysis. A: pERK1/2 detection in TNF-α, SST, seglitide, and L-817,818-treated Caco-2 cells. Data are mean ± SE from 3–5 separate experiments. *P < 0.05 for TNF-α-treated cells (TNF) vs. control cells (CT), SST-treated cells (TNF + SST), seglitide-treated cells (TNF + SEG), and L-817,818-treated cells (TNF + L). B: NHE8 protein expression in TNF-α, SST, and PD98059-treated Caco-2 cells. Data are mean ± SE from 3–6 separate experiments. #P < 0.05 for TNF-α-treated cells (TNF) vs. controls cells (CT), SST-treated cells (TNF + SST), PD98059-treated cells (TNF + PD), and SST/PD98059-treated cells (TNF + SST + PD).