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. 2016 Sep 8;311(5):G785–G793. doi: 10.1152/ajpgi.00181.2015

Fig. 4.

Fig. 4.

Effect of dietary K+ loading on BK channel expression in rat proximal and distal colon. A: Western blots of protein (30 μg per lane) from 3 control distal colons (lanes 1-3) and 3 K+-loaded distal colons (lanes 4-6). B: blots from 3 control proximal colons (lanes 1-3) and 3 K+-loaded proximal colons (lanes 4-6). Blots were repeated in 3 additional control and K+-loaded animals, so that paired proximal and distal colonic segments were obtained from 6 control and 6 K+-loaded animals. After probing with BKα antibody, blots were stripped and probed with anti-GAPDH antibody, GAPDH acting as internal control. Molecular sizes of BKα and GAPDH were ∼120 and 40 kDa, respectively. C and D: summaries of quantified protein band densities from 6 distal colons and 6 proximal colons, respectively. *P < 0.001.