Fig. 6.

NED19-dependent inhibition of Ca²⁺ release. Open box represents saline, and black box indicates duration and concentration of CCh stimulus. Acini were stimulated at a threshold concentration of CCh with (A, red trace) or without (A, black trace) NED19 pretreatment. There was no change in the peak amplitude of Ca²⁺ release following NED19 treatment (0.13 ± 0.038 r.u., n = 10) when compared with control (0.17 ± 0.032 r.u., n = 14, P = 0.44) at a threshold level of stimulation (C). In contrast, following cAMP elevation in the absence (B, black trace) or presence (B, red trace) of NED19 pretreatment, there was a significant reduction in the peak amplitude (ISOP/IBMX, 0.14 ± 0.017 r.u., n = 13 vs. ISOP/IBMX + NED19, 0.088 ± 0.016, n = 13, *P = 0.026) of threshold responses (C).