Fig. 3.

Ca²⁺-dependent activities of MH-associated RyR1 mutants in the presence of 1 mM Mg²⁺. A–H: Ca²⁺-dependent activities with and without 1 mM Mg²⁺ were determined for WT-RyR1, WT-RyR2, and six MH-associated RyR1 mutants. Data of Ca²⁺-dependent activities without 1 mM Mg²⁺ are from Fig. 2. Data are shown as means ± SE (n = 4–8). I: IC₅₀ values of wild-type and mutant RyRs in the presence of 1 mM Mg²⁺ are shown. *P < 0.05 compared with WT-RyR1 by one-way ANOVA followed by Tukey's test among seven sample groups (wild-type and mutant RyR1s). Data are shown as means ± SE (n = 4–8).