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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Int J Parasitol. 2016 Oct 8;46(13-14):871–880. doi: 10.1016/j.ijpara.2016.08.003

Fig. 5.

Fig. 5

Bumped kinase inhibitor (BKI) 1294 is more effective if added at the time of infection rather than 2 h later. Culture-derived merozoites (4 × 104/well) of Sarcocystis neurona-yellow fluorescent protein (YFP) clone F9F seeded onto 96-well monolayers of bovine turbinate (BT) host cells. BKI-1294 was added at the time of parasite inoculation (0 h) and incubated for 2 h. For the second part of the experiment, BKI-1294 was added 2 h p.i.. Wells were washed two times to remove non-invaded S. neurona before adding BKI-1294 back to wells. Plates were incubated at 37°C for 4 days, and fluorescence measured growth inhibition was based on S. neurona-YFP control wells containing no drug. BT cells in 2% serum were used as blank wells. The data above represents the mean of eight wells.