Table 1.

Sarcocystis neurona calcium-dependent protein kinase 1 (SnCDPK1) cell lysate thermal shift and enzyme inhibition with S. neurona cell EC₅₀s in the presence of bumped kinase inhibitors (BKIs).

BKIs	Recombinant SnCDPK1 Thermal Shift (°C) at 20 µM	Recombinant TgCDPK1 Thermal Shift (°C) at 20 µM	S. neurona cell CDPK1 Thermal Stability (°C)	SnCDPK1 enzyme IC50 (µM)	TgCDPK1 enzyme IC50 (µM)	S. neurona cell growth EC50 (µM)
1553	11.2	12.3	4.1	0.009	0.001a	0.042
1266	0.7	1.6	0	>2	0.500b	>10
1294	9.9	12.3	2.7	0.006	0.003c	0.068
1561	9.6	10.6	ND	0.008	0.002a	0.128
1517	9.6	10.5	8.0	0.002	0.002d	0.042

^aVidadala et al., 2016

^bOjo et al., 2012

^cJohnson et al., 2012

^dHuang et al., 2015

Tg, Toxoplasma gondii; IC₅₀, inhibition concentration to give 50% reduction in enzyme activity; ND, not done;

EC₅₀, inhibition concentration to give 50% reduction in cell growth.