FIGURE 1.

Reactive oxygen species detection by confocal laser scanning microscopy in Arabidopsis thaliana cell suspension cultures. Cells were grown in bioreactor in normoxic conditions (T0), subjected to anoxia 2 h (T1) or 4 h (T2) and re-oxygenation 2 h (T3) or 20 h (T4). Merged two-color confocal images (a–e) of the green and the red channel show autofluorescence of chloroplasts (red false color) and nonspecific ROS (green false color) detected by carboxy-H₂DCFDA probe. In (a₁–e₁) the confocal microscopy images are digitally merged with the transmitted light images for displaying the number of labeled cells in the total cell population. Excitation wavelength 488 nm line, emission was detected at 505–530 nm band pass filter and 650 nm long band-pass filter. Scale bar = 50 μm.