FIGURE 2.

Hydroxyl peroxide (H₂O₂) detection by confocal microscopy in A. thaliana cell suspension cultures. Cells were grown in bioreactor in normoxic conditions (T0), subjected to anoxia 2 h (T1) or 4 h (T2) and re-oxygenation 2 h (T3) or 20 h (T4). T2m represents a magnification of T2 cells. Merged two-color confocal images (a–f) of the green and the red channel show H₂O₂ (green false color) detected by DHR probe (R123), and autofluorescence of chloroplasts (red false color). In (a₁–f₁), the confocal microscopy images are digitally merged with the transmitted light images for displaying the number of labeled cells in the total cell population. Excitation wavelength 488 nm line, emission was detected at 505–530 nm band pass filter and 650 nm long band-pass filter. Scale bar = 50 μm.