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. 2016 Dec 1;10:553. doi: 10.3389/fnins.2016.00553

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Copyright © 2016 Mayorova, Tian, Cai, Semmens, Odekunle, Zandawala, Badi, Rowe, Egertová and Elphick.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization of asterotocin precursor transcripts in larvae of the starfish Asterias rubens using whole-mount in situ hybridization. (A,B) bipinnaria larvae, showing no detectable expression of asterotocin precursor transcripts (A, left lateral view; B, frontal view). (C–E) brachiolaria larva. The schematic drawings illustrate the distribution of stained cells (see Figure 1 for labeling). (C) left lateral view, showing expression in the tips of the brachia (black arrowheads) and near the adhesive disk (white arrow). (D) detail of the attachment complex (boxed region in C) showing stained cells in the brachium tips (black arrowheads), the adhesive disk (double arrow), and adjacent to the adhesive disk (white arrow). (E) detail of the attachment complex showing absence of staining in a larva incubated with sense probes. abr, anterior brachium; ad, adhesive disk; ap, anterior projection; ar, adult rudiment; ba, bipinnaria arms; e, esophagus; g, gut; m, mouth; lbr, lateral brachium; pol, postoral lobe; prl, preoral lobe; s, stomach; st, stem of a brachium; t, tip of a brachium. Scale bars: 200 μm (A–C), 100 μm (D,E).