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. 2016 Dec 1;10:553. doi: 10.3389/fnins.2016.00553

Figure 7.

Figure 7

Localization of ArGnRHP transcripts in larvae of the starfish Asterias rubens using whole-mount in situ hybridization. (A) bipinnaria larva, left lateral view, showing no detectable expression of ArGnRHP transcripts; (B–D) brachiolaria larva; the schematic drawings illustrate the distribution of stained cells (see Figure 1 for labeling). (B) left lateral view, showing stained cells close to the adhesive disk (white arrow) (C) detail of the attachment complex (boxed region in B) showing two groups of stained cells near the adhesive disk (white arrows). (D) absence of staining in a brachiolaria larva incubated with sense probes. a, anus; abr, anterior brachium; ad, adhesive disk; ap, anterior projection; ar, adult rudiment; ba, bipinnaria arms; e, esophagus; g, gut; lbr, lateral brachium; prl, preoral lobe; s, stomach; st, stem of a brachium; t, tip of a brachium. Scale bars: 200 μm (A,B,D), 100 μm (C).